Bloody Battle! JoJo & Dio

The group detours to Poco's home and learns that his sister has vanished. She is in truth locked away with Dio, who dangles eternal life before her; when she refuses and slaps him, the vampire storms off and sets the zombie Doobie upon her. Jonathan arrives in time to cut the creature down with ease, then climbs the stairs to the final chamber where Dio waits. He challenges his foe to avenge his slain friends, but Dire shoves him aside to fight in Zeppeli's name. Dio freezes Dire's legs and shatters him, yet the severed head survives long enough to launch a Ripple-charged rose into Dio's right eye.

Jonathan steps up while Speedwagon, Straizo, and Tonpetty hold off the horde of zombies. Inspired by Dire, he pours the Ripple into Luck and Pluck, the sword Bruford gave him, so that Dio dare not touch it. He distracts the vampire with roses, strikes from a blind angle to sever an arm, then cleaves him in two, only for Dio to freeze the blade and his hand. Immobilized, Jonathan uses a nearby fire to heat the sword and break free, but Dio snaps the weapon and ices his fist before the Ripple can land. In a final gamble, Jonathan sets his glove alight to beat the freezing and drives the Ripple into Dio's chest, hurling him into a cliff.

The episode covers Chapter 37 through Chapter 41. As a last spiteful act, Dio unleashes a jet of pressurized eye fluid that nearly kills Jonathan, who still finds the strength to mourn his fallen half-brother before collapsing from exhaustion, victorious. Among the adaptation choices, the anime has Doobie melt rather than be devoured by his own snakes, Speedwagon wields a hammer during the fight, and Dio's eye-fluid attack is exaggerated to pierce the clouds instead of merely cutting a pillar.