Tsukuyomi is a secretive psychic research group in One-Punch Man that abducted and experimented on a young Tatsumaki in pursuit of artificial espers. Framing its work as progress for humanity, it resurfaces years later chasing Psykos, drawing the fury of the grown Tatsumaki.
Tsukuyomi is a psychic research outfit staffed by scientists whose stated aim is advancing humanity through the study of psychokinetic power. In practice, the organization is best remembered for the cruel experiments it carried out on Tatsumaki when she was a child, and it appears to have been working toward the creation of artificial espers at its facilities.
Its true leadership and base of operations are never revealed, and among espers the very name has taken on the flavor of an urban legend. The group is treated as an enemy of Tatsumaki, whose history with it runs deep and bitter.
Years earlier, Tsukuyomi found the seven-year-old Tatsumaki, recognized her gift, and bought her away from her adoptive parents, leaving behind the toddler Fubuki because she had not yet manifested any power. Tatsumaki was then confined and tested for three years until a hybrid monster tore loose inside the compound. Feigning helplessness in her cell, she was saved when Blast arrived and slew the creature. She fled to reach Fubuki, and when four identical agents ambushed her along the way, she is presumed to have killed them, a sight that left a young Fubuki terrified of her sister.
Much later, after the Monster Association collapsed, Tsukuyomi offered the cash-strapped Hero Association a fortune for the captured Psykos, calling her a valuable sample for research into her precognitive third eye. It sent the agent Apollo to collect her, but Tatsumaki and Fubuki intercepted him; though outmatched, he nearly seized all three before escaping with an undercover operative planted in the Blizzard Group. When heroes pressed two Hero Association executives about the deal, Gearsper waved it off as a rumor, guessing the real culprits were Monster Association survivors.
The only named operatives tied to the group are Apollo, the agent dispatched to retrieve Psykos, and an unnamed Tsukuyomi spy who had embedded himself within Fubuki's Blizzard Group. Both are listed as active. The rank and file are otherwise depicted as interchangeable scientists and identical agents, and Psykos herself is hinted to share some buried connection with it, reacting violently whenever the name is spoken.

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Tsukuyomi is a secretive psychic research organization in One-Punch Man whose scientists study psychokinetic power under the stated goal of advancing humanity, though it is best known for the cruel experiments it performed on a young Tatsumaki.
Tsukuyomi abducts and experiments on children with psychic potential in pursuit of creating artificial espers, and it later attempts to acquire the captured esper Psykos from the Hero Association for further research.
Tsukuyomi found the seven-year-old Tatsumaki, recognized her psychic gift, and bought her away from her adoptive parents, leaving behind her toddler sister Fubuki because she had not yet manifested any power.
Tatsumaki was confined and tested for three years until a hybrid monster broke loose inside the facility; she was saved when the hero Blast arrived and killed the creature, allowing her to flee.
The only named Tsukuyomi operatives are Apollo, the agent sent to retrieve Psykos, and an unnamed spy who had infiltrated Fubuki's Blizzard Group.
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