Awakened by Kōzō Fuyutsuki in Evangelion: 3.0+1.0 Thrice Upon A Time, the Advanced Ayanami Series is NERV's next generation of Rei Ayanami clones, pure-souled bodies prepared as offerings to the Vessels of the Adams for the saga's final battle.
Every member of the series is cast from the same mold as Rei Ayanami: cropped blue hair, red eyes, and a distinctly female figure. They first appear as ranks of identical bodies inside NERV headquarters.
Kōzō Fuyutsuki wakes the clones at NERV HQ in Evangelion: 3.0+1.0 Thrice Upon A Time, hailing the line's resurrection and describing them as untainted beings whose pure souls carry no sexual distinction, bred as offerings for the Vessel of the Adams. The film never shows one climbing into an entry plug, but the implication is that they crew Evangelion Mark.09-A, Mark.10, Mark.11, and Mark.12. Those four units open the gates of Guf during the closing confrontation and are destroyed one after another by Unit-08γ, presumably taking their pilots with them.

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The Advanced Ayanami Series is NERV's next generation of Rei Ayanami clones, awakened by Kozo Fuyutsuki in Evangelion: 3.0+1.0 Thrice Upon A Time and bred as offerings for the Vessels of the Adams.
Every member of the Advanced Ayanami Series shares Rei Ayanami's mold: cropped blue hair, red eyes, and a female figure, first shown as ranks of identical bodies inside NERV headquarters.
NERV manufactures the Advanced Ayanami Series to pilot Evangelions and to serve as sacrificial offerings to the Vessels of the Adams during the series' final battle.
The film never shows one of the clones climbing into an entry plug, but it is implied they crew Evangelion Mark.09-A, Mark.10, Mark.11, and Mark.12.
The four Evangelion units associated with the Advanced Ayanami Series open the gates of Guf during the climactic battle and are destroyed one after another by Unit-08 gamma, presumably taking their pilots with them.
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